› 6to2

X-ray diffraction
2Å resolution

Crystal structure of CYP154C5 from Nocardia farcinica in complex with 5alpha-Androstan-3-one

Released:
DOI: 10.2210/pdb6to2/pdb
PDB entry 6to2 coloured by chain, front view.
PDB entry 6to2 coloured by chain, side view.
PDB entry 6to2 coloured by chain, top view.
Source organism: Nocardia farcinica IFM 10152
Primary publication:
CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering*.
Bracco P, Wijma HJ, Nicolai B, Buitrago JAR, Klünemann T, Vila A, Schrepfer P, Blankenfeldt W, Janssen DB, Schallmey A
Chembiochem 22 1099-1110 (2021)
PMID: 33145893

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 45.22 KDa
Source organism: Nocardia farcinica IFM 10152
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q5YNS8 (Residues: 1-409; Coverage: 100%)
Gene name: NFA_53110
Sequence domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand NQ8 2 x NQ8
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 103.411Å b: 103.411Å c: 218.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.249
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Recent trends in biocatalysis.
Yi et al. (2021)
2 other articles

1 mention without citation

CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering*.
Bracco et al. (2021)