X-ray diffraction
1.08Å resolution

Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylorubrum extorquens AM1 in a close conformation containing NADP+ and methylene-H4MPT


Function and Biology Details

Reaction catalysed:
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Bifunctional protein MdtA Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 31.51 KDa
Source organism: Methylorubrum extorquens AM1
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P55818 (Residues: 1-288; Coverage: 100%)
Gene names: MexAM1_META1p1728, mtdA
Sequence domains: Methylene-tetrahydromethanopterin dehydrogenase, N-terminal

Ligands and Environments

Cofactor: Ligand NAP 1 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P4132
Unit cell:
a: 125.754Å b: 125.754Å c: 125.754Å
α: 90° β: 90° γ: 90°
R R work R free
0.114 0.113 0.127
Expression system: Escherichia coli BL21(DE3)