› 6tb2

X-ray diffraction
2.9Å resolution

Structure of human haptoglobin-hemoglobin bound to S. aureus IsdH

Released:
DOI: 10.2210/pdb6tb2/pdb
PDB entry 6tb2 coloured by chain, front view.
PDB entry 6tb2 coloured by chain, side view.
PDB entry 6tb2 coloured by chain, top view.
Source organisms:
Entry authors: Mikkelsen JH, Andersen CBF

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-133188 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemoglobin subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Hemoglobin subunit beta Chain: B
Molecule details ›
Chain: B
Length: 146 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Haptoglobin beta chain Chain: C
Molecule details ›
Chain: C
Length: 267 amino acids
Theoretical weight: 29.79 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00738 (Residues: 148-406; Coverage: 67%)
Gene name: HP
Sequence domains: Trypsin
Iron-regulated surface determinant protein H Chains: D, E
Molecule details ›
Chains: D, E
Length: 354 amino acids
Theoretical weight: 40.97 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2FXJ2 (Residues: 321-655; Coverage: 39%)
Gene names: SAOUHSC_01843, harA, isdH, sasI
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 84.1Å b: 86.77Å c: 217.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.27
Expression systems:
  • Cricetulus griseus
  • Escherichia coli BL21(DE3)

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