› 6t9q

X-ray diffraction
1.15Å resolution

Crystal structure of the second, C-terminal repeat of the DNA-binding domain of human TImeless

Released:
DOI: 10.2210/pdb6t9q/pdb
PDB entry 6t9q coloured by chain, front view.
PDB entry 6t9q coloured by chain, side view.
PDB entry 6t9q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Timeless couples G-quadruplex detection with processing by DDX11 helicase during DNA replication.
Lerner LK, Holzer S, Kilkenny ML, Šviković S, Murat P, Schiavone D, Eldridge CB, Bittleston A, Maman JD, Branzei D, Stott K, Pellegrini L, Sale JE
EMBO J 39 e104185 (2020)
PMID: 32705708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein timeless homolog Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNS1 (Residues: 883-947; Coverage: 5%)
Gene names: TIM, TIM1, TIMELESS, TIMELESS1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P65
Unit cell:
a: 55.407Å b: 55.407Å c: 41.659Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.129 0.128 0.155
Expression system: Escherichia coli

Quick links

Citations

13 review citations

G4-quadruplex-binding proteins: review and insights into selectivity.
Meier-Stephenson V. (2022)
12 more