› 6t8o

Electron Microscopy
3.99Å resolution

Stalled FtsK motor domain bound to dsDNA end

Released:
DOI: 10.2210/pdb6t8o/pdb
PDB entry 6t8o coloured by chain, front view.
PDB entry 6t8o coloured by chain, side view.
PDB entry 6t8o coloured by chain, top view.
Source organisms:
Primary publication:
FtsK in motion reveals its mechanism for double-stranded DNA translocation.
Jean NL, Rutherford TJ, Löwe J
Proc Natl Acad Sci U S A 117 14202-14208 (2020)
PMID: 32513722
Related structures: EMD-10402

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120638 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA translocase FtsK Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 491 amino acids
Theoretical weight: 54 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I0M3 (Residues: 247-728; Coverage: 59%)
Gene names: PA2615, ftsK
Sequence domains:
dsDNA substrate Chains: G, H
Molecule details ›
Chains: G, H
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ADP 6 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.99Å
Relevant EMDB volumes: EMD-10402
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Mechanisms of hexameric helicases.
Fernandez et al. (2021)
7 more

2 mentions without citation

FtsK in motion reveals its mechanism for double-stranded DNA translocation.
Jean et al. (2020)
1 more