X-ray diffraction
1.4Å resolution

Structure of the Bottromycin epimerase BotH in complex with Bottromycin A2


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 33.09 KDa
Source organism: Streptomyces sp. BC16019
Expression system: Escherichia coli BL21(DE3)
  • Canonical: K4MHV9 (Residues: 2-293; Coverage: 100%)
Gene name: botH
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 66.986Å b: 80.109Å c: 89.42Å
α: 90° β: 90° γ: 90°
R R work R free
0.16 0.159 0.182
Expression system: Escherichia coli BL21(DE3)