› 6t3t

X-ray diffraction
2.1Å resolution

Structure of the 4-hydroxy-tetrahydrodipicolinate synthase from the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV

Released:
DOI: 10.2210/pdb6t3t/pdb
PDB entry 6t3t coloured by chain, front view.
PDB entry 6t3t coloured by chain, side view.
PDB entry 6t3t coloured by chain, top view.
Source organism: Methylacidiphilum fumariolicum SolV
Primary publication:
Structure of the 4-hydroxy-tetrahydrodipicolinate synthase from the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV and the phylogeny of the aminotransferase pathway.
Schmitz RA, Dietl A, Müller M, Berben T, Op den Camp HJM, Barends TRM
Acta Crystallogr F Struct Biol Commun 76 199-208 (2020)
PMID: 32356521

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 33.09 KDa
Source organism: Methylacidiphilum fumariolicum SolV
UniProt:
  • Canonical: I0JZ23 (Residues: 1-301; Coverage: 100%)
Gene names: MFUM_700041, dapA
Sequence domains: Dihydrodipicolinate synthetase family

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P42
Unit cell:
a: 109.06Å b: 109.06Å c: 107.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.232

Quick links

Citations

1 review citation

Verrucomicrobial methanotrophs: ecophysiology of metabolically versatile acidophiles.
Schmitz et al. (2021)
2 other articles

1 mention without citation

Structure of the 4-hydroxy-tetrahydrodipicolinate synthase from the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV and the phylogeny of the aminotransferase pathway.
Schmitz et al. (2020)