X-ray diffraction
2.6Å resolution

Structure of Thermococcus litoralis Delta(1)-pyrroline-2-carboxylate reductase in complex with NADH and L-proline


Function and Biology Details

Reaction catalysed:
L-pipecolate + NADP(+) = 1-piperideine-2-carboxylate + NADPH 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
DELTA1-pyrroline-2-carboxylate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 38.27 KDa
Source organism: Thermococcus litoralis DSM 5473
Expression system: Escherichia coli
  • Canonical: H3ZMH3 (Residues: 1-209; Coverage: 95%)
Gene name: OCC_00362

Ligands and Environments

Cofactor: Ligand NAI 2 x NAI
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21
Unit cell:
a: 82.688Å b: 51.509Å c: 92.29Å
α: 90° β: 115.906° γ: 90°
R R work R free
0.191 0.189 0.242
Expression system: Escherichia coli