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Bat Influenza A polymerase recycling complex

Electron Microscopy
3.52Å resolution
PDB entry 6t2c coloured by chain, front view.
PDB entry 6t2c coloured by chain, side view.
PDB entry 6t2c coloured by chain, top view.
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Assembly composition:
hetero tetramer (preferred)
Entry contents: 3 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 51881.4 Å2
Buried surface area: 29196.85 Å2
Dissociation area: 3,115.95 Å2
Dissociation energy (ΔGdiss): 59.83 kcal/mol
Dissociation entropy (TΔSdiss): 13.06 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124826

Macromolecules

Polymerase acidic protein
Chain: A
Length: 738 amino acids
Theoretical weight: 85.49 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM92 (Residues: 1-713; Coverage: 100%)
Gene name: PA
Pfam: Influenza RNA-dependent RNA polymerase subunit PA
InterPro:
Polymerase acidic protein in PDB entry 6t2c, assembly 1, front view.
Polymerase acidic protein in PDB entry 6t2c, assembly 1, side view.
Polymerase acidic protein in PDB entry 6t2c, assembly 1, top view.
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RNA-directed RNA polymerase catalytic subunit
Chain: B
Length: 776 amino acids
Theoretical weight: 87.94 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM91 (Residues: 1-756; Coverage: 100%)
Gene name: PB1
Pfam: Influenza RNA-dependent RNA polymerase subunit PB1
InterPro:
RNA-directed RNA polymerase catalytic subunit in PDB entry 6t2c, assembly 1, front view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 6t2c, assembly 1, side view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 6t2c, assembly 1, top view.
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Polymerase basic protein 2
Chain: C
Length: 809 amino acids
Theoretical weight: 91.03 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM90 (Residues: 1-760; Coverage: 100%)
Gene name: PB2
Pfam:
InterPro:
Polymerase basic protein 2 in PDB entry 6t2c, assembly 1, front view.
Polymerase basic protein 2 in PDB entry 6t2c, assembly 1, side view.
Polymerase basic protein 2 in PDB entry 6t2c, assembly 1, top view.
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RNA chain V
Name: vRNA
Representative chains: V
Length: 34 nucleotides
Theoretical weight: 10.84 KDa
vRNA in PDB entry 6t2c, assembly 1, front view.
vRNA in PDB entry 6t2c, assembly 1, side view.
vRNA in PDB entry 6t2c, assembly 1, top view.
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