› 6t23

Electron Microscopy
3.1Å resolution

Cryo-EM structure of jasplakinolide-stabilized F-actin (aged)

Released:
DOI: 10.2210/pdb6t23/pdb
PDB entry 6t23 coloured by chain, front view.
PDB entry 6t23 coloured by chain, side view.
PDB entry 6t23 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural Effects and Functional Implications of Phalloidin and Jasplakinolide Binding to Actin Filaments.
Pospich S, Merino F, Raunser S
Structure 28 437-449.e5 (2020)
PMID: 32084355
Related structures: EMD-10365

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

4 bound ligands:
Ligand ADP 5 x ADP
Ligand PO4 5 x PO4
Ligand MG 5 x MG
Ligand 9ZK 5 x 9ZK
1 modified residue:
Ligand HIC 5 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-10365

Quick links

Citations

6 review citations

Towards a structural understanding of the remodeling of the actin cytoskeleton.
Merino et al. (2020)
5 more

3 mentions without citation

Chemical Biology Tools To Investigate Malaria Parasites.
Broichhagen et al. (2021)
2 more