6t13

X-ray diffraction
1.85Å resolution

CRYSTAL STRUCTURE OF GLUCOCEREBROSIDASE IN COMPLEX WITH A PYRROLOPYRAZINE

Released:
Source organism: Homo sapiens
Entry authors: Benz J, Ehler A, Hug M, Huber S, Rufer AC, Guba W, Jagasia R, Hofmann EC, Rodriguez Sarmiento RM

Function and Biology Details

Reaction catalysed:
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal acid glucosylceramidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 536 amino acids
Theoretical weight: 59.76 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04062 (Residues: 1-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 90.849Å b: 135.408Å c: 191.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.204
Expression system: Cricetulus griseus