› 6syr

X-ray diffraction
1.49Å resolution

The wild type glucuronoyl esterase OtCE15A from Opitutus terrae in complex with D-glucuronate

Released:
DOI: 10.2210/pdb6syr/pdb
PDB entry 6syr coloured by chain, front view.
PDB entry 6syr coloured by chain, side view.
PDB entry 6syr coloured by chain, top view.
Source organism: Opitutus terrae PB90-1
Primary publication:
Structural and biochemical studies of the glucuronoyl esterase OtCE15A illuminate its interaction with lignocellulosic components.
Mazurkewich S, Poulsen JN, Lo Leggio L, Larsbrink J
J Biol Chem 294 19978-19987 (2019)
PMID: 31740581

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative acetyl xylan esterase Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 47.84 KDa
Source organism: Opitutus terrae PB90-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1ZMF4 (Residues: 1-437; Coverage: 100%)
Gene name: Oter_0116

Ligands and Environments

4 bound ligands:
Ligand GCU 1 x GCU
Ligand MG 1 x MG
Ligand EDO 4 x EDO
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P1
Unit cell:
a: 43.215Å b: 44.189Å c: 50.172Å
α: 75.776° β: 65.425° γ: 70.847°
R-values:
R R work R free
0.186 0.183 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glucuronoyl esterases - enzymes to decouple lignin and carbohydrates and enable better utilization of renewable plant biomass.
Larsbrink et al. (2023)
1 more

3 mentions without citation

Structural and biochemical studies of the glucuronoyl esterase OtCE15A illuminate its interaction with lignocellulosic components.
Mazurkewich et al. (2019)
2 more