6swr

X-ray diffraction
3.2Å resolution

Crystal structure of the lysosomal potassium channel MtTMEM175 T38A mutant soaked with zinc

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-122951 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 486 amino acids
Theoretical weight: 53.27 KDa
Source organism: Lama glama
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: P0AEX9 (Residues: 32-395; Coverage: 98%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Potassium channel Ftrac_2467 Chains: B, E
Molecule details ›
Chains: B, E
Length: 255 amino acids
Theoretical weight: 29.45 KDa
Source organism: Marivirga tractuosa DSM 4126
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: E4TN31 (Residues: 2-247; Coverage: 100%)
Gene name: Ftrac_2467
Sequence domains: Endosomal/lysosomal potassium channel TMEM175

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Unit cell:
a: 129.788Å b: 131.611Å c: 151.771Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.269 0.299
Expression system: Escherichia coli MC1061