› 6svf

X-ray diffraction
1.6Å resolution

Crystal structure of the P235GK mutant of ArgBP from T. maritima

Released:
DOI: 10.2210/pdb6svf/pdb
PDB entry 6svf coloured by chain, front view.
PDB entry 6svf coloured by chain, side view.
PDB entry 6svf coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
The non-swapped monomeric structure of the arginine-binding protein from Thermotoga maritima.
Smaldone G, Ruggiero A, Balasco N, Abuhammad A, Autiero I, Caruso D, Esposito D, Ferraro G, Gelardi ELM, Moreira M, Quareshy M, Romano M, Saaret A, Selvam I, Squeglia F, Troisi R, Kroon-Batenburg LMJ, Esposito L, Berisio R, Vitagliano L
Acta Crystallogr F Struct Biol Commun 75 707-713 (2019)
PMID: 31702584

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amino acid ABC transporter, periplasmic amino acid-binding protein Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.52 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZ62 (Residues: 20-234, 235-246; Coverage: 92%)
Gene name: TM_0593
Sequence domains: Bacterial extracellular solute-binding proteins, family 3

Ligands and Environments

1 bound ligand:
Ligand ARG 1 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 45.72Å b: 52.964Å c: 95.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.218
Expression system: Escherichia coli

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Citations

1 citations in other articles

Development of a Protein Scaffold for Arginine Sensing Generated through the Dissection of the Arginine-Binding Protein from Thermotoga maritima.
Smaldone et al. (2020)

2 mentions without citation

The non-swapped monomeric structure of the arginine-binding protein from Thermotoga maritima.
Smaldone et al. (2019)
1 more