› 6sul

X-ray diffraction
1.35Å resolution

Amicoumacin kinase AmiN in complex with AMP-PNP, Mg2+ and Ami

Released:
DOI: 10.2210/pdb6sul/pdb
PDB entry 6sul coloured by chain, front view.
PDB entry 6sul coloured by chain, side view.
PDB entry 6sul coloured by chain, top view.
Source organism: Bacillus pumilus
Primary publication:
A kinase bioscavenger provides antibiotic resistance by extremely tight substrate binding.
Terekhov SS, Mokrushina YA, Nazarov AS, Zlobin A, Zalevsky A, Bourenkov G, Golovin A, Belogurov A, Osterman IA, Kulikova AA, Mitkevich VA, Lou HJ, Turk BE, Wilmanns M, Smirnov IV, Altman S, Gabibov AG
Sci Adv 6 eaaz9861 (2020)
PMID: 32637600

Function and Biology Details

Reaction catalysed: 
ATP + amicoumacin A = ADP + amicoumacin A 2-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 335 amino acids
Theoretical weight: 39.11 KDa
Source organism: Bacillus pumilus
Expression system: Escherichia coli
UniProt:
  • Canonical: A8FAR5 (Residues: 1-335; Coverage: 100%)
Gene name: BPUM_0640
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

4 bound ligands:
Ligand MG 8 x MG
Ligand CL 10 x CL
Ligand UAM 4 x UAM
Ligand ANP 4 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P1
Unit cell:
a: 45.34Å b: 76.77Å c: 101.2Å
α: 89.95° β: 99.62° γ: 91.44°
R-values:
R R work R free
0.151 0.149 0.188
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Novel Enzymes From the Red Sea Brine Pools: Current State and Potential.
Renn et al. (2021)
1 more