6spo

X-ray diffraction
1.2Å resolution

Structure of the Escherichia coli methionyl-tRNA synthetase complexed with methionine

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 568 amino acids
Theoretical weight: 64.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0F3U9S7 (Residues: 2-548; Coverage: 81%)
Gene names: BvCmsKSP058_01266, BvCmsNSP007_01600, C9Z23_20390, ED648_21370, UN91_27160, metG
Sequence domains: tRNA synthetases class I (M)

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 78.29Å b: 45.19Å c: 85.89Å
α: 90° β: 107.39° γ: 90°
R-values:
R R work R free
0.138 0.136 0.167
Expression system: Escherichia coli