› 6snj

Solution NMR

Solution structure of the FUS/TLS RNA recognition motif in complex with U1 snRNA stem loop III

Released:
DOI: 10.2210/pdb6snj/pdb
PDB entry 6snj coloured by chain, ensemble of 18 models, front view.
PDB entry 6snj coloured by chain, ensemble of 18 models, side view.
PDB entry 6snj coloured by chain, ensemble of 18 models, top view.
Source organism: Homo sapiens
Primary publication:
Aberrant interaction of FUS with the U1 snRNA provides a molecular mechanism of FUS induced amyotrophic lateral sclerosis.
Jutzi D, Campagne S, Schmidt R, Reber S, Mechtersheimer J, Gypas F, Schweingruber C, Colombo M, von Schroetter C, Loughlin FE, Devoy A, Hedlund E, Zavolan M, Allain FH, Ruepp MD
Nat Commun 11 6341 (2020)
PMID: 33311468

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152981 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein FUS Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35637 (Residues: 260-390; Coverage: 25%)
Gene names: FUS, TLS
Sequence domains: RNA recognition motif
U1 snRNA stem loop III, RNA (28-MER) Chain: B
Molecule details ›
Chain: B
Length: 28 nucleotides
Theoretical weight: 8.95 KDa
Source organism: Homo sapiens
Expression system: in vitro transcription vector pT7-Fluc(deltai)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 80%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • in vitro transcription vector pT7-Fluc(deltai)

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Citations

5 review citations

The Interplay of RNA Binding Proteins, Oxidative Stress and Mitochondrial Dysfunction in ALS.
Harley et al. (2021)
4 more

4 mentions without citation

Principles and correction of 5'-splice site selection.
Malard et al. (2022)
3 more