› 6smz

X-ray diffraction
1.75Å resolution

Crystal structure of SLA Reductase YihU from E. Coli in complex with NADH

Released:
DOI: 10.2210/pdb6smz/pdb
PDB entry 6smz coloured by chain, front view.
PDB entry 6smz coloured by chain, side view.
PDB entry 6smz coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Dynamic Structural Changes Accompany the Production of Dihydroxypropanesulfonate by Sulfolactaldehyde Reductase
Sharma M, Abayakoon P, Lingford JP, Epa R, John A, Jin Y, Goddard-Borger ED, Davies GJ, Williams SJ
ACS Catalysis (2020)

Function and Biology Details

Reactions catalysed: 
(2S)-2,3-dihydroxypropane-1-sulfonate + NAD(+) = (2S)-2-hydroxy-3-oxopropane-1-sulfonate + NADH
4-hydroxybutanoate + NAD(+) = succinate semialdehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-sulfolactaldehyde reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 306 amino acids
Theoretical weight: 32.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9V8 (Residues: 1-298; Coverage: 100%)
Gene names: JW3853, b3882, yihU
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 72.512Å b: 113.743Å c: 142.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.2
Expression system: Escherichia coli BL21(DE3)

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