6sm0

X-ray diffraction
1.91Å resolution

Venus 66 p-Azido-L-Phenylalanin (azF) variant, dark grown

Released:
Source organism: Aequorea victoria
Entry authors: Rizkallah PJ, Al Maslookhi HS, Jones DD

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.69 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-230; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 50.947Å b: 63.251Å c: 69.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.243
Expression system: Escherichia coli