6slo

X-ray diffraction
1.94Å resolution

Crystal structure of PUF60 UHM domain in complex with 7,8 dimethoxyperphenazine

Released:
Entry authors: Jagtap PKA, Kubelka T, Bach T, Sattler M

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-binding-splicing factor PUF60; Thioredoxin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 222 amino acids
Theoretical weight: 24.67 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHX1 (Residues: 460-559; Coverage: 18%)
  • Canonical: A0A064CXU8 (Residues: 1-109; Coverage: 100%)
Gene names: AF42_05360, FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: Thioredoxin

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 81.52Å b: 89.4Å c: 149.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.207
Expression system: Escherichia coli BL21(DE3)