› 6sjo

X-ray diffraction
1.95Å resolution

Methyltransferase of the MtgA D102A mutant from Desulfitobacterium hafniense in complex with methyl-tetrahydrofolate

Released:
DOI: 10.2210/pdb6sjo/pdb
PDB entry 6sjo coloured by chain, front view.
PDB entry 6sjo coloured by chain, side view.
PDB entry 6sjo coloured by chain, top view.
Source organism: Desulfitobacterium hafniense DCB-2
Primary publication:
Structures in Tetrahydrofolate Methylation in Desulfitobacterial Glycine Betaine Metabolism at Atomic Resolution.
Badmann T, Groll M
Chembiochem 21 776-779 (2020)
PMID: 31518049

Function and Biology Details

Reaction catalysed: 
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(In) = 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na(+)(Out)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrahydromethanopterin S-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.46 KDa
Source organism: Desulfitobacterium hafniense DCB-2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8FUR2 (Residues: 2-306; Coverage: 100%)
Gene name: Dhaf_4327
Sequence domains: Tetrahydromethanopterin S-methyltransferase MtrH subunit

Ligands and Environments

2 bound ligands:
Ligand THH 1 x THH
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 77.03Å b: 84.29Å c: 87.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structures in Tetrahydrofolate Methylation in Desulfitobacterial Glycine Betaine Metabolism at Atomic Resolution.
Badmann et al. (2020)