6sjc

X-ray diffraction
2.23Å resolution

Structure of T. thermophilus AspRS in Complex with 5'-O-(N-(L-aspartyl)-sulfamoyl)adenosine

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate--tRNA(Asp) ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 581 amino acids
Theoretical weight: 66.18 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36419 (Residues: 1-580; Coverage: 100%)
Gene name: aspS
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 82.089Å b: 112.764Å c: 88.073Å
α: 90° β: 104.17° γ: 90°
R-values:
R R work R free
0.188 0.187 0.232
Expression system: Escherichia coli BL21(DE3)