› 6sj9

X-ray diffraction
2.2Å resolution

Proteasome accessory factor B/C (PafBC) of Arthrobacter aurescens

Released:
DOI: 10.2210/pdb6sj9/pdb
PDB entry 6sj9 coloured by chain, front view.
PDB entry 6sj9 coloured by chain, side view.
PDB entry 6sj9 coloured by chain, top view.
Source organism: Paenarthrobacter aurescens
Primary publication:
Structure and functional implications of WYL domain-containing bacterial DNA damage response regulator PafBC.
Müller AU, Leibundgut M, Ban N, Weber-Ban E
Nat Commun 10 4653 (2019)
PMID: 31604936

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WYL domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 664 amino acids
Theoretical weight: 73.27 KDa
Source organism: Paenarthrobacter aurescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A4Y3NDN0 (Residues: 18-681; Coverage: 97%)
Gene name: AAU01_20320
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand K 2 x K
Ligand SCN 2 x SCN
Ligand PG4 1 x PG4
Ligand PEG 4 x PEG
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 77.875Å b: 119.033Å c: 160.212Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Survival in Hostile Conditions: Pupylation and the Proteasome in Actinobacterial Stress Response Pathways.
von Rosen et al. (2021)
9 other articles

4 mentions without citation

A widespread family of WYL-domain transcriptional regulators co-localizes with diverse phage defence systems and islands.
Picton et al. (2022)
3 more