› 6sj2

X-ray diffraction
1.25Å resolution

Amidohydrolase, AHS with 3-HAA

Released:
DOI: 10.2210/pdb6sj2/pdb
PDB entry 6sj2 coloured by chain, front view.
PDB entry 6sj2 coloured by chain, side view.
PDB entry 6sj2 coloured by chain, top view.
Source organism: Streptomyces sp. Tu 6176
Primary publication:
The Biosynthesis of the Benzoxazole in Nataxazole Proceeds via an Unstable Ester and has Synthetic Utility.
Song H, Rao C, Deng Z, Yu Y, Naismith JH
Angew Chem Int Ed Engl 59 6054-6061 (2020)
PMID: 31903677

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 56.4 KDa
Source organism: Streptomyces sp. Tu 6176
Expression system: Streptomyces albus
UniProt:
  • Canonical: A0A022MQ12 (Residues: 1-494; Coverage: 100%)
Gene names: CF54_07360, natAM
Sequence domains: Amidohydrolase family

Ligands and Environments

4 bound ligands:
Ligand 3HA 2 x 3HA
Ligand ZN 2 x ZN
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21212
Unit cell:
a: 99.81Å b: 118.89Å c: 83.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.178
Expression system: Streptomyces albus

Quick links

Citations

1 review citation

Aryl Coenzyme A Ligases, a Subfamily of the Adenylate-Forming Enzyme Superfamily.
Arnold et al. (2021)
6 other articles