› 6sgs

Electron Microscopy
3.2Å resolution

Cryo-EM structure of Escherichia coli AcrBZ and DARPin in Saposin A-nanodisc

Released:
DOI: 10.2210/pdb6sgs/pdb
PDB entry 6sgs coloured by chain, front view.
PDB entry 6sgs coloured by chain, side view.
PDB entry 6sgs coloured by chain, top view.
Source organisms:
Primary publication:
Interactions of a Bacterial RND Transporter with a Transmembrane Small Protein in a Lipid Environment.
Du D, Neuberger A, Orr MW, Newman CE, Hsu PC, Samsudin F, Szewczak-Harris A, Ramos LM, Debela M, Khalid S, Storz G, Luisi BF
Structure 28 625-634.e6 (2020)
PMID: 32348749
Related structures: EMD-10183

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151950 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli str. K-12 substr. MG1655
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
DARPin Chains: D, E
Molecule details ›
Chains: D, E
Length: 169 amino acids
Theoretical weight: 18.32 KDa
Source organism: synthetic construct
Expression system: Escherichia coli str. K-12 substr. MG1655
Multidrug efflux pump accessory protein AcrZ Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 49 amino acids
Theoretical weight: 5.3 KDa
Source organism: Shigella boydii 965-58
Expression system: Escherichia coli str. K-12 substr. MG1655

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-10183
Expression system: Escherichia coli str. K-12 substr. MG1655

Quick links

Citations

16 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
15 more

2 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
1 more