6sge

X-ray diffraction
1.5Å resolution

Crystal structure of Human RHOB-GTP in complex with nanobody B6

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho-related GTP-binding protein RhoB Chains: A, C
Molecule details ›
Chains: A, C
Length: 183 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62745 (Residues: 1-183; Coverage: 93%)
Gene names: ARH6, ARHB, RHOB
Sequence domains: Ras family
Nanobody B6 Chains: B, D
Molecule details ›
Chains: B, D
Length: 134 amino acids
Theoretical weight: 14.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 66.84Å b: 70.227Å c: 71.212Å
α: 90° β: 107.68° γ: 90°
R-values:
R R work R free
0.162 0.161 0.188
Expression system: Escherichia coli