6sc8

X-ray diffraction
2.11Å resolution

Function and Biology Details

Reaction catalysed:
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase RNF31 Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 43.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96EP0 (Residues: 697-1072; Coverage: 35%)
Gene names: RNF31, ZIBRA
Sequence domains:
Single domain antibody Chains: B, C
Molecule details ›
Chains: B, C
Length: 120 amino acids
Theoretical weight: 13.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I222
Unit cell:
a: 65.919Å b: 87.77Å c: 241.831Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.261
Expression system: Escherichia coli