› 6sbf

X-ray diffraction
1.3Å resolution

Structure of type II terpene cyclase MstE_Y157F from Scytonema (apo)

Released:
DOI: 10.2210/pdb6sbf/pdb
PDB entry 6sbf coloured by chain, front view.
PDB entry 6sbf coloured by chain, side view.
PDB entry 6sbf coloured by chain, top view.
Source organism: Scytonema sp. PCC 10023
Primary publication:
A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.
Moosmann P, Ecker F, Leopold-Messer S, Cahn JKB, Dieterich CL, Groll M, Piel J
Nat Chem 12 968-972 (2020)
PMID: 32778689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Squalene cyclase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 40.64 KDa
Source organism: Scytonema sp. PCC 10023
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2D1CM82 (Residues: 2-366; Coverage: 100%)
Gene name: mstE
Sequence domains: Squalene-hopene cyclase C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 47.81Å b: 77.41Å c: 91.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.116 0.115 0.147
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Assembly-Line Catalysis in Bifunctional Terpene Synthases.
Faylo et al. (2021)
2 more

1 mention without citation

A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.
Moosmann et al. (2020)