› 6sb3

Electron Microscopy
3.5Å resolution

CryoEM structure of murine perforin-2 ectodomain in a pre-pore form

Released:
DOI: 10.2210/pdb6sb3/pdb
PDB entry 6sb3 coloured by chain, front view.
PDB entry 6sb3 coloured by chain, side view.
PDB entry 6sb3 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunity.
Ni T, Jiao F, Yu X, Aden S, Ginger L, Williams SI, Bai F, Pražák V, Karia D, Stansfeld P, Zhang P, Munson G, Anderluh G, Scheuring S, Gilbert RJC
Sci Adv 6 eaax8286 (2020)
PMID: 32064340
Related structures: EMD-10134

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage-expressed gene 1 protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 646 amino acids
Theoretical weight: 71.13 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: A1L314 (Residues: 20-652; Coverage: 91%)
Gene name: Mpeg1
Sequence domains: MAC/Perforin domain

Ligands and Environments

1 bound ligand:
Ligand NAG 32 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-10134
Expression system: Homo sapiens

Quick links

Citations

9 review citations

To Kill But Not Be Killed: Controlling the Activity of Mammalian Pore-Forming Proteins.
Krawczyk et al. (2020)
8 more

6 mentions without citation

Ancient but Not Forgotten: New Insights Into MPEG1, a Macrophage Perforin-Like Immune Effector.
Bayly-Jones et al. (2020)
5 more