6s7t

Electron Microscopy
3.5Å resolution

Cryo-EM structure of human oligosaccharyltransferase complex OST-B

Released:
Source organism: Homo sapiens
Related structures: EMD-10112

Function and Biology Details

Reaction catalysed:
Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (13 distinct):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B Chain: A
Molecule details ›
Chain: A
Length: 826 amino acids
Theoretical weight: 93.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8TCJ2 (Residues: 1-826; Coverage: 100%)
Gene names: SIMP, STT3B
Sequence domains: Oligosaccharyl transferase STT3 subunit
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 37 amino acids
Theoretical weight: 4.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C6T2 (Residues: 1-37; Coverage: 100%)
Gene name: OST4
Sequence domains: Oligosaccaryltransferase
Transmembrane protein 258 Chain: C
Molecule details ›
Chain: C
Length: 79 amino acids
Theoretical weight: 9.08 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61165 (Residues: 1-79; Coverage: 100%)
Gene names: C11orf10, HSPC005, TMEM258
Sequence domains: Oligosaccharyltransferase subunit 5
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Chain: D
Molecule details ›
Chain: D
Length: 113 amino acids
Theoretical weight: 12.5 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61803 (Residues: 1-113; Coverage: 100%)
Gene name: DAD1
Sequence domains: DAD family
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Chain: E
Molecule details ›
Chain: E
Length: 607 amino acids
Theoretical weight: 68.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P04843 (Residues: 1-607; Coverage: 100%)
Gene name: RPN1
Sequence domains: Ribophorin I
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 Chain: F
Molecule details ›
Chain: F
Length: 631 amino acids
Theoretical weight: 69.35 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P04844 (Residues: 1-631; Coverage: 100%)
Gene name: RPN2
Sequence domains: Oligosaccharyltransferase subunit Ribophorin II
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Chain: G
Molecule details ›
Chain: G
Length: 456 amino acids
Theoretical weight: 50.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P39656 (Residues: 1-456; Coverage: 100%)
Gene names: DDOST, KIAA0115, OK/SW-cl.45, OST48
Sequence domains: Oligosaccharyltransferase 48 kDa subunit beta
Magnesium transporter protein 1 Chain: H
Molecule details ›
Chain: H
Length: 335 amino acids
Theoretical weight: 38.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H0U3 (Residues: 1-335; Coverage: 100%)
Gene names: IAG2, MAGT1, PSEC0084, UNQ628/PRO1244
Sequence domains: OST3 / OST6 family, transporter family
Malectin Chain: I
Molecule details ›
Chain: I
Length: 292 amino acids
Theoretical weight: 32.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q14165 (Residues: 1-292; Coverage: 100%)
Gene names: KIAA0152, MLEC
Sequence domains: Malectin domain
PEPTIDE Chain: K
Molecule details ›
Chain: K
Length: 7 amino acids
Theoretical weight: 589 Da
Source organism: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-10112
Expression system: Homo sapiens