6s7k

X-ray diffraction
1.55Å resolution

Fumarate hydratase of Mycobacterium tuberculosis in complex with formate and allosteric modulator N-(2-Methoxy-5-(N-methylsulfamoyl)phenyl)-2-(4-oxo-3,4-dihydrophthalazin-1-yl)acetamide

Released:

Function and Biology Details

Reaction catalysed:
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase class II Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 474 amino acids
Theoretical weight: 50.19 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WN93 (Residues: 1-474; Coverage: 100%)
Gene names: MTV017.51c, Rv1098c, fum, fumC
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 174.669Å b: 96.388Å c: 123.707Å
α: 90° β: 102.65° γ: 90°
R-values:
R R work R free
0.172 0.171 0.195
Expression system: Escherichia coli BL21(DE3)