6s6t

Electron Microscopy
4.1Å resolution

Structure of Azospirillum brasilense Glutamate Synthase in a4b3 oligomeric state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutamate synthase [NADPH] large chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1515 amino acids
Theoretical weight: 166.22 KDa
Source organism: Azospirillum brasilense
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05755 (Residues: 1-1515; Coverage: 100%)
Gene name: gltB
Sequence domains:
Glutamate synthase [NADPH] small chain Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 482 amino acids
Theoretical weight: 52.43 KDa
Source organism: Azospirillum brasilense
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05756 (Residues: 1-482; Coverage: 100%)
Gene name: gltD
Sequence domains:

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN

Cofactor: Ligand FAD 3 x FAD
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-10105
Expression system: Escherichia coli