› 6s4a

X-ray diffraction
1.95Å resolution

Function and Biology Details

Reactions catalysed: 
5,10-methylenetetrahydrofolate + NAD(+) = 5,10-methenyltetrahydrofolate + NADH
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 34.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13995 (Residues: 36-350; Coverage: 90%)
Gene names: MTHFD2, NMDMC
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand KUK 2 x KUK
Ligand PO4 6 x PO4
Ligand MPD 7 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P65
Unit cell:
a: 115.068Å b: 115.068Å c: 113.549Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.211
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Targeting the DNA damage response and repair in cancer through nucleotide metabolism.
Helleday et al. (2022)
2 more

3 mentions without citation

Pharmacological targeting of MTHFD2 suppresses acute myeloid leukemia by inducing thymidine depletion and replication stress.
Bonagas et al. (2022)
2 more