6s01

Electron Microscopy
3.2Å resolution

Structure of LEDGF PWWP domain bound H3K36 methylated nucleosome

Released:
Source organisms:
Related structures: EMD-10069

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 135 amino acids
Theoretical weight: 15.33 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 2-136; Coverage: 99%)
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 129 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 2-130; Coverage: 99%)
Sequence domains:
Histone H2B 1.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 122 amino acids
Theoretical weight: 13.52 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
PC4 and SFRS1-interacting protein Chain: K
Molecule details ›
Chain: K
Length: 530 amino acids
Theoretical weight: 60.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75475 (Residues: 1-530; Coverage: 100%)
Gene names: DFS70, LEDGF, PSIP1, PSIP2
Sequence domains:
Wisdom 601 DNA (165-MER) Chain: I
Molecule details ›
Chain: I
Length: 165 nucleotides
Theoretical weight: 50.7 KDa
Wisdom 601 DNA (165-MER) Chain: J
Molecule details ›
Chain: J
Length: 165 nucleotides
Theoretical weight: 51.17 KDa

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-10069
Expression systems:
  • Escherichia coli
  • Trichoplusia ni