› 6rx8

X-ray diffraction
1.92Å resolution

EDDS lyase variant D290M/Y320M with bound fumarate

Released:
DOI: 10.2210/pdb6rx8/pdb
PDB entry 6rx8 coloured by chain, front view.
PDB entry 6rx8 coloured by chain, side view.
PDB entry 6rx8 coloured by chain, top view.
Source organism: Chelativorans sp. BNC1
Primary publication:
Engineered C-N Lyase: Enantioselective Synthesis of Chiral Synthons for Artificial Dipeptide Sweeteners.
Zhang J, Grandi E, Fu H, Saravanan T, Bothof L, Tepper PG, Thunnissen AWH, Poelarends GJ
Angew Chem Int Ed Engl 59 429-435 (2020)
PMID: 31625664

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
argininosuccinate lyase Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 55.74 KDa
Source organism: Chelativorans sp. BNC1
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q11KV9 (Residues: 1-502; Coverage: 100%)
Gene name: Meso_0564
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FUM 2 x FUM
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: F222
Unit cell:
a: 143.16Å b: 144.23Å c: 144.451Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.221
Expression system: Escherichia coli K-12

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Citations

2 review citations

Biocatalysis: Enzymatic Synthesis for Industrial Applications.
Wu et al. (2021)
1 more

1 mention without citation

Engineered C-N Lyase: Enantioselective Synthesis of Chiral Synthons for Artificial Dipeptide Sweeteners.
Zhang et al. (2020)