› 6rqr

X-ray diffraction
2.2Å resolution

Extended NHERF1 PDZ2 domain in complex with the PDZ-binding motif of CFTR

Released:
DOI: 10.2210/pdb6rqr/pdb
PDB entry 6rqr coloured by chain, front view.
PDB entry 6rqr coloured by chain, side view.
PDB entry 6rqr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
In vivo crystals reveal critical features of the interaction between cystic fibrosis transmembrane conductance regulator (CFTR) and the PDZ2 domain of Na+/H+ exchange cofactor NHERF1.
Martin ER, Barbieri A, Ford RC, Robinson RC
J Biol Chem 295 4464-4476 (2020)
PMID: 32014995

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystic fibrosis transmembrane conductance regulator; Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13569 (Residues: 1101-1101; Coverage: 0%)
  • Canonical: O14745 (Residues: 150-269; Coverage: 34%)
Gene names: ABCC7, CFTR, NHERF, NHERF1, SLC9A3R1
Sequence domains: PDZ domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: I212121
Unit cell:
a: 84.95Å b: 85.039Å c: 89.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.206 0.233
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Noncanonical Sequences Involving NHERF1 Interaction with NPT2A Govern Hormone-Regulated Phosphate Transport: Binding Outside the Box.
Mamonova et al. (2021)
3 more

1 mention without citation

In vivo crystals reveal critical features of the interaction between cystic fibrosis transmembrane conductance regulator (CFTR) and the PDZ2 domain of Na+/H+ exchange cofactor NHERF1.
Martin et al. (2020)