6rqf

Electron Microscopy
3.58Å resolution

3.6 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Spinacia oleracea with natively bound thylakoid lipids and plastoquinone molecules

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Cytochrome b6 Chains: A, I
Molecule details ›
Chains: A, I
Length: 215 amino acids
Theoretical weight: 24.19 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P00165 (Residues: 1-215; Coverage: 100%)
Gene name: petB
Sequence domains: Cytochrome b/b6/petB
Cytochrome b6-f complex subunit 4 Chains: B, J
Molecule details ›
Chains: B, J
Length: 160 amino acids
Theoretical weight: 17.46 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P00166 (Residues: 1-160; Coverage: 100%)
Gene name: petD
Sequence domains: Cytochrome b(C-terminal)/b6/petD
Cytochrome f Chains: C, K
Molecule details ›
Chains: C, K
Length: 285 amino acids
Theoretical weight: 31.36 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P16013 (Residues: 36-320; Coverage: 100%)
Gene name: petA
Sequence domains:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic Chains: D, L
Molecule details ›
Chains: D, L
Length: 179 amino acids
Theoretical weight: 18.96 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P08980 (Residues: 52-230; Coverage: 78%)
Gene name: petC
Sequence domains: Rieske [2Fe-2S] domain
Cytochrome b6-f complex subunit 6 Chains: E, M
Molecule details ›
Chains: E, M
Length: 31 amino acids
Theoretical weight: 3.45 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: Q9M3L0 (Residues: 1-31; Coverage: 100%)
Gene name: petL
Sequence domains: Cytochrome B6-F complex subunit VI (PetL)
Cytochrome b6-f complex subunit 7 Chains: F, N
Molecule details ›
Chains: F, N
Length: 36 amino acids
Theoretical weight: 3.77 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P80883 (Residues: 1-36; Coverage: 100%)
Gene name: petM
Sequence domains: PetM family of cytochrome b6f complex subunit 7
Cytochrome b6-f complex subunit 5 Chains: G, O
Molecule details ›
Chains: G, O
Length: 37 amino acids
Theoretical weight: 4.17 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P69461 (Residues: 1-37; Coverage: 100%)
Gene names: petE, petG
Sequence domains: Cytochrome B6-F complex subunit 5
Cytochrome b6-f complex subunit 8 Chains: H, P
Molecule details ›
Chains: H, P
Length: 29 amino acids
Theoretical weight: 3.17 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P61045 (Residues: 1-29; Coverage: 100%)
Gene names: petN, ycf6
Sequence domains: PetN

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.58Å
Relevant EMDB volumes: EMD-4981