6rko

Electron Microscopy
2.68Å resolution

Cryo-EM structure of the E. coli cytochrome bd-I oxidase at 2.68 A resolution

Released:

Function and Biology Details

Reaction catalysed:
(1a) 2 ubiquinol = 2 ubiquinone + 4 H(+)(Side 1) + 4 e(-)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cytochrome bd-I ubiquinol oxidase subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 379 amino acids
Theoretical weight: 42.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABK2 (Residues: 1-379; Coverage: 100%)
Gene names: JW0723, b0734, cyd-2, cydB
Sequence domains: Cytochrome bd terminal oxidase subunit II
Cytochrome bd-I ubiquinol oxidase subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 58.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABJ9 (Residues: 1-522; Coverage: 100%)
Gene names: JW0722, b0733, cyd-1, cydA
Sequence domains: Cytochrome bd terminal oxidase subunit I
Uncharacterized protein YnhF Chain: H
Molecule details ›
Chain: H
Length: 29 amino acids
Theoretical weight: 3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: A5A618 (Residues: 1-29; Coverage: 100%)
Gene names: JW1649.1, b4602, ynhF
Cytochrome bd-I ubiquinol oxidase subunit X Chain: X
Molecule details ›
Chain: X
Length: 37 amino acids
Theoretical weight: 4.04 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P56100 (Residues: 1-37; Coverage: 100%)
Gene names: JW0724, b4515, cydX, ybgT
Sequence domains: Membrane bound YbgT-like protein

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.68Å
Relevant EMDB volumes: EMD-4908
Expression system: Escherichia coli