› 6rfx

X-ray diffraction
1.9Å resolution

Crystal structure of Eis2 from Mycobacterium abscessus

Released:
DOI: 10.2210/pdb6rfx/pdb
PDB entry 6rfx coloured by chain, front view.
PDB entry 6rfx coloured by chain, side view.
PDB entry 6rfx coloured by chain, top view.
Source organism: Mycobacteroides abscessus
Primary publication:
Crystal structure of the aminoglycosides N-acetyltransferase Eis2 from Mycobacterium abscessus.
Ung KL, Alsarraf HMAB, Olieric V, Kremer L, Blaise M
FEBS J 286 4342-4355 (2019)
PMID: 31254444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enhanced intracellular survival protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 411 amino acids
Theoretical weight: 45.5 KDa
Source organism: Mycobacteroides abscessus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1M9A4M7 (Residues: 2-411; Coverage: 100%)
Gene names: SAMEA2070793_03201, SAMEA2071181_03011, SAMEA2259684_02562, SAMEA2275847_00725, eis, eis_1, eis_2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CIT 6 x CIT
Ligand P4K 7 x P4K
Ligand ACT 5 x ACT
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 112.45Å b: 79.8Å c: 152.68Å
α: 90° β: 90.29° γ: 90°
R-values:
R R work R free
0.203 0.202 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Non-tuberculous mycobacteria and the rise of Mycobacterium abscessus.
Johansen et al. (2020)
2 more

1 mention without citation

Crystal structure of the aminoglycosides N-acetyltransferase Eis2 from Mycobacterium abscessus.
Ung et al. (2019)