› 6rbw

X-ray diffraction
2.5Å resolution

Crystal structure of NAD kinase 1 from Listeria monocytogenes in complexe with an adenine derivative

Released:
DOI: 10.2210/pdb6rbw/pdb
PDB entry 6rbw coloured by chain, front view.
PDB entry 6rbw coloured by chain, side view.
PDB entry 6rbw coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
From Substrate to Fragments to Inhibitor Active In Vivo against Staphylococcus aureus.
Gelin M, Paoletti J, Nahori MA, Huteau V, Leseigneur C, Jouvion G, Dugué L, Clément D, Pons JL, Assairi L, Pochet S, Labesse G, Dussurget O
ACS Infect Dis 6 422-435 (2020)
PMID: 32017533

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 31.05 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Y8D7 (Residues: 1-264; Coverage: 100%)
Gene names: lmo0968, nadK1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand JXB 1 x JXB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: I222
Unit cell:
a: 62.273Å b: 76.568Å c: 118.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.238 0.299
Expression system: Escherichia coli

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Citations

1 review citation

CRISPR-Based Approaches for Gene Regulation in Non-Model Bacteria.
Call et al. (2022)
5 other articles