› 6rb0

X-ray diffraction
2.35Å resolution

STRUCTURE OF ESTER-HYDROLASE EH1AB1 FROM THE METAGENOME OF LAKE ARREO COMPLEXED WITH A DERIVATIVE OF METHYL 4-NITROPHENYL HEXYLPHOSPHONATE

Released:
DOI: 10.2210/pdb6rb0/pdb
PDB entry 6rb0 coloured by chain, front view.
PDB entry 6rb0 coloured by chain, side view.
PDB entry 6rb0 coloured by chain, top view.
Source organism: metagenome
Primary publication:
Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis
Alonso S, Santiago G, Cea-Rama I, Fernandez-Lopez L, Coscolin C, Modregger J, Ressmann AK, Martinez-Martinez M, Marrero H, Bargiela R, Pita M, Gonzalez-Alfonso JL, Briand ML, Rojo D, Barbas C, Plou FJ, Golyshin PN, Shahgaldian P, Sanz-Aparicio J, Guallar V, Ferrer M
Nat Catal 3 (2020)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EH1AB1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 35.6 KDa
Source organism: metagenome
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand MHH 4 x MHH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 83.077Å b: 86.09Å c: 97.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.212
Expression system: Escherichia coli BL21(DE3)

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