6raw

Electron Microscopy
3.7Å resolution

D. melanogaster CMG-DNA, State 1A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Entry contents:
11 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (13 distinct):
DNA replication licensing factor Mcm2 Chain: 2
Molecule details ›
Chain: 2
Length: 887 amino acids
Theoretical weight: 100.54 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49735 (Residues: 1-887; Coverage: 100%)
Gene names: CG7538, Mcm2
Sequence domains:
DNA replication licensing factor Mcm3 Chain: 3
Molecule details ›
Chain: 3
Length: 819 amino acids
Theoretical weight: 91.05 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9XYU1 (Residues: 1-819; Coverage: 100%)
Gene names: CG4206, Mcm3, Mcm3-RA
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 866 amino acids
Theoretical weight: 96.74 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q26454 (Residues: 1-866; Coverage: 100%)
Gene names: CG1616, dpa
Sequence domains:
DNA replication licensing factor Mcm5 Chain: 5
Molecule details ›
Chain: 5
Length: 733 amino acids
Theoretical weight: 82.38 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VGW6 (Residues: 1-733; Coverage: 100%)
Gene names: CG4082, Mcm5
Sequence domains:
DNA replication licensing factor Mcm6 Chain: 6
Molecule details ›
Chain: 6
Length: 817 amino acids
Theoretical weight: 92.47 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9V461 (Residues: 1-817; Coverage: 100%)
Gene names: CG4039, Mcm6
Sequence domains:
DNA replication licensing factor Mcm7 Chain: 7
Molecule details ›
Chain: 7
Length: 720 amino acids
Theoretical weight: 81.4 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9XYU0 (Residues: 1-720; Coverage: 100%)
Gene names: CG4978, Mcm7
Sequence domains:
CDC45L Chain: A
Molecule details ›
Chain: A
Length: 575 amino acids
Theoretical weight: 65.97 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O96989 (Residues: 1-575; Coverage: 100%)
Gene names: CDC45, CDC45L, CG3658, Cdc45, D, DmCdc45, Dmel\CG3658, Dmel_CG3658, EG:BACR7A4.11, anon-1Ec, cdc45, dCDC45, dCDC45L
Sequence domains: CDC45-like protein
IP07275p Chain: H
Molecule details ›
Chain: H
Length: 202 amino acids
Theoretical weight: 23.33 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W0I7 (Residues: 1-202; Coverage: 100%)
Gene names: CG9187, CG9187-PA, Dmel\CG9187, Dmel_CG9187, PSF1, Psf1, psf1
Probable DNA replication complex GINS protein PSF2 Chain: L
Molecule details ›
Chain: L
Length: 203 amino acids
Theoretical weight: 23.14 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VQY9 (Residues: 1-203; Coverage: 100%)
Gene names: CG18013, Psf2
Sequence domains: GINS complex protein
Sld5 domain-containing protein Chain: M
Molecule details ›
Chain: M
Length: 212 amino acids
Theoretical weight: 24.83 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W2V7 (Residues: 1-212; Coverage: 100%)
Gene names: CG2222, Dmel\CG2222, Dmel_CG2222, PSF3, Psf3
Sequence domains: GINS complex protein
DNA replication complex GINS protein SLD5 Chain: N
Molecule details ›
Chain: N
Length: 228 amino acids
Theoretical weight: 26.15 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VBI1 (Residues: 1-228; Coverage: 100%)
Gene names: CG14549, Dmel\CG14549, Dmel_CG14549, SLD5, Sld5, anon-WO0172774.61
Sequence domains:
DNA Chain: F
Molecule details ›
Chain: F
Length: 26 nucleotides
Theoretical weight: 7.93 KDa
DNA (5'-D(P*TP*CP*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 13 nucleotides
Theoretical weight: 3.97 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-4785
Expression system: Trichoplusia ni