› 6r9p

X-ray diffraction
2.98Å resolution

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AAUUAA RNA

Released:
DOI: 10.2210/pdb6r9p/pdb
PDB entry 6r9p coloured by chain, front view.
PDB entry 6r9p coloured by chain, side view.
PDB entry 6r9p coloured by chain, top view.
Source organisms:
Primary publication:
The intrinsic structure of poly(A) RNA determines the specificity of Pan2 and Caf1 deadenylases.
Tang TTL, Stowell JAW, Hill CH, Passmore LA
Nat Struct Mol Biol 26 433-442 (2019)
PMID: 31110294

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156695 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Chain: A
Molecule details ›
Chain: A
Length: 672 amino acids
Theoretical weight: 77.78 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53010 (Residues: 461-1115; Coverage: 59%)
Gene names: PAN2, YGL094C
Sequence domains:
Structure domains: Cysteine proteinases
AAUUAA RNA Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I222
Unit cell:
a: 91.885Å b: 117.091Å c: 255.888Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.261 0.309
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

11 review citations

Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.
Passmore et al. (2022)
10 more

2 mentions without citation

The intrinsic structure of poly(A) RNA determines the specificity of Pan2 and Caf1 deadenylases.
Tang et al. (2019)
1 more