› 6r3e

X-ray diffraction
2.27Å resolution

Human DNMT3B PWWP domain in complex with triisopropanolamine

Released:
DOI: 10.2210/pdb6r3e/pdb
PDB entry 6r3e coloured by chain, front view.
PDB entry 6r3e coloured by chain, side view.
PDB entry 6r3e coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Rondelet G, Dal Maso T, Wouters J

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 3B Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 30.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBC3 (Residues: 215-351; Coverage: 21%)
Gene name: DNMT3B
Sequence domains: PWWP domain

Ligands and Environments

2 bound ligands:
Ligand 96H 1 x 96H
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3221
Unit cell:
a: 74.257Å b: 74.257Å c: 158.187Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.218 0.264
Expression system: Escherichia coli BL21(DE3)

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