› 6qzz

X-ray diffraction
1.85Å resolution

full length OphA V404E in complex with SAH

Released:
DOI: 10.2210/pdb6qzz/pdb
PDB entry 6qzz coloured by chain, front view.
PDB entry 6qzz coloured by chain, side view.
PDB entry 6qzz coloured by chain, top view.
Source organism: Omphalotus olearius
Primary publication:
Substrate Plasticity of a Fungal Peptide α-N-Methyltransferase.
Song H, Fahrig-Kamarauskaitè JR, Matabaro E, Kaspar H, Shirran SL, Zach C, Pace A, Stefanov BA, Naismith JH, Künzler M
ACS Chem Biol 15 1901-1912 (2020)
PMID: 32491837

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104401 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methyltransferase/ribosomally synthesized cyclic peptide omphalotin A precursor ophMA Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 45.68 KDa
Source organism: Omphalotus olearius
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A2R2JFI5 (Residues: 3-417; Coverage: 100%)
Gene name: ophMA
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Methyltransferase/ribosomally synthesized cyclic peptide omphalotin A precursor ophMA Chain: B
Molecule details ›
Chain: B
Length: 416 amino acids
Theoretical weight: 45.71 KDa
Source organism: Omphalotus olearius
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A2R2JFI5 (Residues: 3-417; Coverage: 100%)
Gene name: ophMA
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
2 modified residues:
Ligand MVA 3 x MVA
Ligand EME 1 x EME

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 164.002Å b: 91.723Å c: 85.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
1 more

1 mention without citation

Substrate Plasticity of a Fungal Peptide α-N-Methyltransferase.
Song et al. (2020)