› 6qzr

X-ray diffraction
2.3Å resolution

14-3-3 sigma in complex with FOXO1 pT24 peptide

Released:
DOI: 10.2210/pdb6qzr/pdb
PDB entry 6qzr coloured by chain, front view.
PDB entry 6qzr coloured by chain, side view.
PDB entry 6qzr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
AMPK and AKT protein kinases hierarchically phosphorylate the N-terminus of the FOXO1 transcription factor, modulating interactions with 14-3-3 proteins.
Saline M, Badertscher L, Wolter M, Lau R, Gunnarsson A, Jacso T, Norris T, Ottmann C, Snijder A
J Biol Chem 294 13106-13116 (2019)
PMID: 31308176

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152154 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Forkhead box protein O1 Chains: J, M, N, O, P, R, T, U
Molecule details ›
Chains: J, M, N, O, P, R, T, U
Length: 11 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q12778 (Residues: 19-29; Coverage: 2%)
Gene names: FKHR, FOXO1, FOXO1A

Ligands and Environments

2 bound ligands:
Ligand B3P 4 x B3P
Ligand GOL 7 x GOL
2 modified residues:
Ligand CSO 8 x CSO
Ligand TPO 8 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21
Unit cell:
a: 149.205Å b: 62.806Å c: 154.044Å
α: 90° β: 103.24° γ: 90°
R-values:
R R work R free
0.223 0.221 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Lipolysis: cellular mechanisms for lipid mobilization from fat stores.
Grabner et al. (2021)
12 more

3 mentions without citation

AMPK and AKT protein kinases hierarchically phosphorylate the N-terminus of the FOXO1 transcription factor, modulating interactions with 14-3-3 proteins.
Saline et al. (2019)
2 more