› 6qwq

X-ray diffraction
1.9Å resolution

Structure of gtPebB

Released:
DOI: 10.2210/pdb6qwq/pdb
PDB entry 6qwq coloured by chain, front view.
PDB entry 6qwq coloured by chain, side view.
PDB entry 6qwq coloured by chain, top view.
Source organism: Guillardia theta CCMP2712
Primary publication:
Crystal structure of the first eukaryotic bilin reductase GtPEBB reveals a flipped binding mode of dihydrobiliverdin.
Sommerkamp JA, Frankenberg-Dinkel N, Hofmann E
J Biol Chem 294 13889-13901 (2019)
PMID: 31366727

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin bilin reductase plastid Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 32.1 KDa
Source organism: Guillardia theta CCMP2712
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L1IWQ9 (Residues: 84-356; Coverage: 81%)
Gene name: GUITHDRAFT_96430
Sequence domains: Ferredoxin-dependent bilin reductase

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P62
Unit cell:
a: 109.6Å b: 109.6Å c: 47.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.211 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

The master regulator MAT1-1-1 of fungal mating binds to its targets via a conserved motif in the human pathogen Aspergillus fumigatus.
Ramšak et al. (2021)
2 more

1 mention without citation

Crystal structure of the first eukaryotic bilin reductase GtPEBB reveals a flipped binding mode of dihydrobiliverdin.
Sommerkamp et al. (2019)