› 6qwo

X-ray diffraction
2Å resolution

Zinc-reconstituted ODP from T. maritima

Released:
DOI: 10.2210/pdb6qwo/pdb
PDB entry 6qwo coloured by chain, front view.
PDB entry 6qwo coloured by chain, side view.
PDB entry 6qwo coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
A di-iron protein recruited as an Fe[II] and oxygen sensor for bacterial chemotaxis functions by stabilizing an iron-peroxy species.
Muok AR, Deng Y, Gumerov VM, Chong JE, DeRosa JR, Kurniyati K, Coleman RE, Lancaster KM, Li C, Zhulin IB, Crane BR
Proc Natl Acad Sci U S A 116 14955-14960 (2019)
PMID: 31270241

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 29.2 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9S5W7 (Residues: 1-253; Coverage: 100%)
Gene name: TM_0013
Sequence domains: ODP family beta lactamase

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 46.433Å b: 121.709Å c: 159.531Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Protein Activity Sensing in Bacteria in Regulating Metabolism and Motility.
Alvarado et al. (2019)
5 more

1 mention without citation

A di-iron protein recruited as an Fe[II] and oxygen sensor for bacterial chemotaxis functions by stabilizing an iron-peroxy species.
Muok et al. (2019)