› 6qvs

X-ray diffraction
1.6Å resolution

Unliganded structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1)

Released:
DOI: 10.2210/pdb6qvs/pdb
PDB entry 6qvs coloured by chain, front view.
PDB entry 6qvs coloured by chain, side view.
PDB entry 6qvs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unliganded and CMP-Neu5Ac bound structures of human α-2,6-sialyltransferase ST6Gal I at high resolution.
Harrus D, Harduin-Lepers A, Glumoff T
J Struct Biol 212 107628 (2020)
PMID: 32971290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactoside alpha-2,6-sialyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 31.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15907 (Residues: 132-406; Coverage: 68%)
Gene names: SIAT1, ST6GAL1
Sequence domains: Glycosyltransferase family 29 (sialyltransferase)

Ligands and Environments

2 bound ligands:
Ligand PEG 2 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 138.816Å b: 94.369Å c: 64.472Å
α: 90° β: 94.31° γ: 90°
R-values:
R R work R free
0.182 0.181 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Sialidase and Sialyltransferase Inhibitors: Targeting Pathogenicity and Disease.
Bowles et al. (2021)
1 more

1 mention without citation

The vertebrate sialylation machinery: structure-function and molecular evolution of GT-29 sialyltransferases.
Harduin-Lepers A. (2023)