› 6qs8

Electron Microscopy
3.9Å resolution

ClpB (DWB and K476C mutant) bound to casein in presence of ATPgammaS - state KC-2B

Released:
DOI: 10.2210/pdb6qs8/pdb
PDB entry 6qs8 coloured by chain, front view.
PDB entry 6qs8 coloured by chain, side view.
PDB entry 6qs8 coloured by chain, top view.
Source organisms:
Primary publication:
Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor.
Deville C, Franke K, Mogk A, Bukau B, Saibil HR
Cell Rep 27 3433-3446.e4 (2019)
PMID: 31216466
Related structures: EMD-4627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159086 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein ClpB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 858 amino acids
Theoretical weight: 95.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P63284 (Residues: 1-857; Coverage: 100%)
Gene names: JW2573, b2592, clpB, htpM
Sequence domains:
casein Chain: S
Molecule details ›
Chain: S
Length: 24 amino acids
Theoretical weight: 2.06 KDa
Source organism: Bos taurus

Ligands and Environments

3 bound ligands:
Ligand AGS 10 x AGS
Ligand MG 8 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-4627
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The molecular principles governing the activity and functional diversity of AAA+ proteins.
Puchades et al. (2020)
9 more

2 mentions without citation

Stairway to translocation: AAA+ motor structures reveal the mechanisms of ATP-dependent substrate translocation.
Gates et al. (2020)
1 more